cytokine flow cytometry

Calman Prussin (Calman_Prussin@d10.niaid.pc.niaid.nih.gov)
Wed, 09 Nov 94 19:43:33 EST

I missed ISAC, but heard there were some data shown demonstrating
intracellular staining for cytokines.

If anyone saw this and would like to comment on:

1. Cytokines detected. IL-4,IL-5 or IL-10?

2. Cells examined. PBMC or ?

3. Methods. The usual PFA and saponin?

4. What was used to stimulate the cells.

4. Quality of data. Did it look believable? What were the controls. How
much noise and nonspecific binding?

Thanks,

Calman Prussin
Allergic Diseases Section
National Institute of Allergy and Infectious Diseases
National Institutes of Health
Bethesda, Maryland U.S.A.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu