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Thank you
Kris Weber
>The formulae I use on our FACStarPLUS to calculate sort rate & "expected"
recovery (in a spread sheet) are:
R = (ma)*exp(-mnT)
where R = sort rate
m = flow rate
a = percent positive
n = number of drops deflected
T = 1/Period (@40 microseconds with a 70 micron nozzle)
exp = exponent ( 10^(-mnT) will work too)
"Expected" recovery is
P = exp(-(-1-a)uTn)
where u = frequency
At 1,200 chromosomes/s, 1% pure, 2 drops, frequency of 25,000; sort rate will be
@ 10/s with 14% recovery.
You'd need to start with over 4 x 10^8 cells!!!
I should also mention that I've only sorted whole cells!
Dennis
*****************************************************************************
* *
* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego e-mail: djyoung@ucsd.edu *
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>Subject: chromosome yield
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