Fixed HIV+ cells run on a Facstar

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To: I1215117--IBMMAIL

SUBJECT: Fixed HIV+ cells run on a Facstar

Is it safe to analyze paraformaldehyde-fixed lymphocytes from HIV
positive patients using a Facstar PLUS which is not in a containment
facility? I have been advised that once cells are fixed in 1.0%
paraformaldehyde and are incubated for 1.0 hour they are not
biohazardous. I'd like feedback on this since the jet-in-air design of
the Facstar forms an aerosol.

Thanks a bunch,

Marie Iannone
Burroughs Wellcome Co.
Research Triangle Park, NC 27709
(919) 315-8709
usbwc8rc@ibmmail.com

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu