antiPCNA-fitc

Rochelle A. Diamond (diamond@cco.caltech.edu)
Fri, 4 Nov 1994 09:56:51 -0800 (PST)

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I am posting this for a friend who does not have an infobahn onramp in
her vicinity. So please respond back to me and I will relay the info.
Thanks.

Rochelle Diamond
Caltech Flow Cytometry Facility
diamond@cco.caltech.edu

Does anyone have any experience using DAKO's anti-PCNA-fitc in rat liver
cells? I am experiencing difficulty with inappropriate G1 staining in
these cells. We are using triton x-100 to wash out free antibody. This
system seems to work in human cells but upon switching to rat liver cells
there is a problem.

Any help or suggestions would gratefully be accepted.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu