ETOH fix

Peter Lopez (PLOPEZ@sorter.dfci.harvard.edu)
Tue, 4 Oct 1994 12:57:38 -0400 (EDT)

Hello,

Does anyone have any tips on how not to lose cells after
ETOH fixation . I have heard from a number of our users that they
start out fixing 2x10E6 cells/tube and see a pellet after the
first spin, but after a long procedure (like Oncor Apoptag) they
seem to have almost nothing left. They use polypropylene tubes, and
have notice a slight increase in retained cells by using FCS in the
wash media, but the numbers are still terrible. Im pretty sure the
loss is in the centrifuge steps- any suggestions?

Peter Lopez

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Peter A. Lopez (617)632-3179 (voice)
Dana Farber Cancer Institute (617)632-3139 (voice)
Core Flow Cytometry Facility NO FAX!
44 Binney St. Room J-312
Boston,MA 02115 PLOPEZ@sorter.dfci.harvard.edu

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