Re: facs bulliten board
Harbor Branch Oceanographic Institution (harbolon@CLASS.ORG)
Fri, 2 Sep 1994 06:09:15 -0700 (PDT)
Hi Tom. We have been routinely using an EPICS Elite with a two-laser
configuration to measure calcium uptake of T-hybridoma cells using
Indo-1AM loading. Both lasers are air cooled;the argon is 15 mw and the
UV source is an Omnichrome HeCd air-cooled 20 mw. We use the HeCd to
measure the short wavelength (a 440 Dichroic with a 385 band pass) and
the argon to measure the long (a 550 dichroic with a 525 band pass).
This system utilizes the gated amp configuration with the two lasers
being non-colinear (wow what a phrase). We were somewhat concerned
initially about the possible "noisy" signals of the HeCd, but much to our
surprise and delight, we get excellent calcium "rise" histograms using
this set-up. Additionally, we use this HeCd for DNA analyses and have
been quite pleased with the results. I would be happy to give you the
complete details (filter set up, etc.) if you are interested.
--Ross--
Ross E. Longley, Ph.D. * Harbor Branch Oceanographic Inst., Inc.
Immunology Group Leader * 5600 U.S. Highway #1, North
Div. Biomed. Marine Res. * Fort Pierce, FL 34946
Phone (407) 465-2400, 486
FAX (407) 465-1523
e-Mail harbolon@class.org
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
