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FLOW CYTOMETRY COURSE
University of Cambridge
19 - 23 September 1994
Course Organisers: Dr M G Ormerod, Reigate
Dr N P Carter, Dept of Pathology,
University of Cambridge
BASIC COURSE: Monday 19 - Wednesday 21 September 1994
ADVANCED COURSE: Wednesday 21 - Friday 23 September 1994
The two courses - basic and advanced - will run sequentially to
give the potential student the choice of attending either or both
of the courses. It is anticipated that, with the generous co-
operation of the manufacturers, Becton-Dickinson, Coulter and
Ortho, there will be at least three bench top cytometers for use
by the students and one machine equipped with two lasers for the
advanced course.
Basic Course
The basic course will assume little prior experience and take the
student through the most important applications. Although it
will fill the needs of someone working in a research environment,
it will have a slight clinical bias.
Topics covered will include: The basics of flow cytometry;
lasers; fluorescence, fluorochromes and optical filters; data
analysis, including handling multiparametric data; detection of
cell surface antigens; DNA analysis, including ploidy and cell
cycle analysis; antigens associated with cell proliferation; cell
cycle analysis of tumours using BrdU; flow cytometry in a
clinical environment.
Practicals will include: three colour immunofluorescent analysis
of human peripheral blood lymphocytes; measurement of a DNA
histogram from paraffin-embedded tissue and; cell cycle analysis
of tumours using the BrdU-anti-BrdU technique. Advanced Course
The advanced students will join the last day of the basic course.
Topics covered will include: cell cycle analysis using BrdU-anti-
BrdU and using BrdU-Hoechst-propidium iodide; chromosome analysis
and sorting; applications in cell and molecular biology;
apoptosis; multi-drug resistance in tumours; measuring enzyme
kinetics.
Practicals and practical demonstrations will include: cell cycle
analysis of tumours using BrdU-anti-BrdU techniques; preparation
and analysis of chromosomes (bivariate and univariate);
intracellular enzyme kinetics (esterases and glutathione-S-
transferase); measurement of intracellular pH and calcium ions;
the measurement of cell cycle kinetics using the BrdU-Hoechst-
propidium iodide method and; the interation of fluorchromes and
DNA.
For further details, please contact Miss Rebecca Morden, The
Royal Microscopical Society, 37/38 St Clements, Oxford, OX4 1AJ,
United Kingdom. Telephone +44 (0)865 248768, Fax +44 (0)865
791237, email RMS@VAX.OX.UK.AC. If sending an email, please
include your full name and address. This well-established course generally
attracts a good attendance, so please book early to avoid disappointment.
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