Bead CV's
Howard Shapiro (HPANDA@HARVARDA.HARVARD.EDU)
Mon, 06 Jun 94 20:35:46 EST
Bead CV's vary considerably with manufacturer, manufacturing process, and
lot number, among other things. Over the years, the beads I have found to
consistently have the lowest CV's have been 2 micron green spheres from
Polysciences, which come out around 1.2 % in scatter and fluorescence in
the best systems on the best days. I've gotten 1.2 % in a Cytopup with
arc lamp illumination at 436 nm, and in laser systems, including one with
a 441 nm, 100 mW HeCd laser. If the lamp or laser noise is 1% RMS, the CV
won't be any lower than that, in the absence of saturation and bleaching
effects. Not all bead lots are that good.
--Howard
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
