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I have a contact without access to the internet who would
appreciate some feedback to the following problem:
They are trying to utilize the Quantum Simply Cellular Beads
(henceforth referred to as QSCB) from Flow Cytometry Standards
Corporation (FCSC) to estimate binding sites on a variety of
cell types. The problem so far has been that each time they add
a conjugated antibody the negative peak moves up and there is
insufficient separation between the negative and first peak.
Theoretically the negative (of the five beads in the QSCB, only
four have antibody binding capacity) should be in the same place
before and after staining. This has happened with several
antibodies of differing affinities, both from homegrown and
commercial preparations, as well as in the presence of 10% BSA
as recommended by FCSC. I offered to post this memo to the net
and accept any feedback from individuals who might have something
to add whether it be advice or similar experiences. Please do
not hesitate to comment if you have anything to add. You can either
email to the flow cytometry mailing list or to me directly
(kelley@mrc.com) and I will pass the info along to the interested
party. Thanks for your input!
Keith
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Keith A. Kelley kelley@mrc.com *
Institute for Research Technologies * A wink is as good
Miles Research Center * as a nod to a
400 Morgan Lane Ph 203-937-2872 * blind horse.
West Haven CT 06516 FAX 203-927-6923 *
*
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