Cell cycle stats in formalin fixed tissue

CJGROS@ccmail.monsanto.com
Wed, 09 Mar 94 16:04:14 CST

We are requesting help in the resolution of a technical problem. We are
attempting to study cell cycle kinetics using nuclei extracted from
formalin fixed-paraffin embedded rat liver tissue. The method works well
and good quality histograms are generated. The problem is that the
estimated S-phase in normal livers is 10-15 percent using B/D's RFIT software.
In fresh, ethanol fixed liver tissue the S-phase is 1-2 percent. So the
questions are:

1. What is the most likely reason for the disparity in the data ?

2. Is it appropriate to use the S-phase data from formalin fixed liver nuclei
as a control value in spite of the fact that it is higher than reported values
for fresh tissue?

Thanks for your help.

Cindy Gross/Dennis Flaherty E-mail: cjgros@ccmail.monsanto.com
Monsanto EHL
St. Louis, MO
Phone: 314/694-7948
FAX: 314/694-7938

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