Fluidics
Need to have cells in suspension flow in single file through an illuminated volume
In most instruments, accomplished by injecting sample into a sheath fluid as it passes through a small (50-300 µm) orifice
When conditions are right, sample fluid flows in a central core that does not mix with the sheath fluid
This is termed Laminar flow (Sheath Flow Principle)
The introduction of a large volume into a small volume in such a way that it becomes “focused” along an axis is called Hydrodynamic Focusing
Notes:
This slide covers the key words that are involved in fluidics.
