FCS Assistant & FCSPress

 
Contributor: Ray Hicks

Affiliation:

University of Cambridge, Department of Medicine
URL: http://www.fcspress.com
email: rh208@cus.cam.ac.uk
 

FCS Assistant is a utility program that is intended to aid analysis of flow cytometric data, stored in the FCS1.0 or FCS2.0 file format. It has a range of routines, some or all of which are missing from commercial analysis applications:
  • Calculation of the Ratio of two parameters
  • Addition of a calculated time parameter
  • Calculation and export of time-variant data for kinetic analysis or quality control
  • Removal of surplus parameters
  • Editing of saved text
  • Conversion of IBM format files to macintosh
  • Conversion of FCS1.0 to FCS2.0
  • Export of raw data (for dot plots and further manipulation)
  • Calculation and export of histogram data

FCSPress is a Macintosh program for analysing and editing Flow Cytometry Data. It lets you:

  • Plot a variety of high resolution graphs
  • Edit and annotate your graphs "on the page"
  • RGB-Gate your data
  • Generate a wide range of statistics
  • Copy for pasting directly into other programs, or save to disc as standard PICT files
  • Calculate the ratio of two parameters - eg for Indo-1 or Fluo3/Fura Red calcium studies.
  • Scale data, or calculate the sum, product or difference of two parameters.
  • Add a calculated time parameter for kinetic studies (eg pH or Calcium).
  • Delete superfluous parameters.
  • Combine many small files into one large one.
  • Compensate data off-line - ideal for multi-beam instruments with no cross-beam compensation
  • Convert older FCS1.0 files and FCS2.0 files to a common FCS2.0 format
  • Export your data as text for plotting in spreadsheets etc
  • Calculate averages,percent responders, standard error etc for kinetic studies - plot selected data versus time
  • Edit text in your file - correct mis-labelling

Click here to download the demo of FCS Assistant.

Click here to download the demo of FCSPress.