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Hewlett-Packard (HP) computers sold with FACS instruments were often bundled with Hewlett-Packard Paintjet color printers. These printers typically have only an IEEE-488 GPIB connection (rather than the standard Centronics parallel printer connection expected by a PC). Nevertheless, if you have an IEEE-488 GPIB card in your PC (see HPPCLINK), you can not only transer files between the HP and PC, but you can print directly from the PC to the Paintjet. Here you can get an ASCII text file, PAINT488.TXT, containing detailed instructions on how to do this.

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The Purdue Utilities are a family of programs which include the following:

GENP takes a two parameter correlated FCS histogram, and generates a list of 'points' and 'weights' from it. It is designed to generate a list of scatterplot points to be read by some other analysis, statistics, or graphics program.
GENP documentation.

KEYS is used to generate a listing of all the keyword-value pairs in an FCS file. This includes the FCS TEXT and ANALYSIS segments, but not any private segments. [MFI displays text block keyword-value pairs to the screen from a point-and-shoot menu of filenames; it does not display the analysis block.]
FCSKEYS is an updated version that runs only under a Win32 DOS prompt.
KEYS documentation.

LDATA reads an FCS histogram file and writes its raw data segment to the screen or to an ASCII file. It is used when it is convenient to have histogram data in a human readable format, or when the data needs to be read by a standard analysis or statistics program. [MFI can write a gated, smoothed ASCII histogram data file from the list mode file.]
LDATA32 is an updated version that runs only under a Win32 DOS prompt.
LDATA documentation.

LLDATA is used to generate a text listing of the data in a listmode file. [MFI can produce a gated ASCII file of list mode data from an FCS list mode file.]
LLDATA32 is an updated version that runs only under a Win32 DOS prompt.
LLDATA documentation.

LOOKUP opens FCS files, and retrieves specific keyword values.
LOOKUP32 is an updated version that runs only under a Win32 DOS prompt.
LOOKUP documentation.

SETKEY is used to change the value of a keyword in an FCS file or to add new keywords to the file. [MFI can change values of keywords which are not critical to data integrity, or add new keywords and values.]
SETKEY documentation.

Authors: J. Paul Robinson and Steve Kelley, Purdue Univ., West Lafayette IN USA. kelley@flowcyt.cyto.purdue.edu.

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(John Curtin School)

This family of utilities includes FCSVIEW, a simple program to plot histograms out specifically on a HP pen plotter (in HPGL). Another utility program reverses the byte order of the binary listmode data in FCS1.0 files.

Inquire from Geoffrey Osborne, John Curtin School of Medical Research, Australian National University, Canberra geoff.osborne@anu.edu.au.

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A macintosh utility to convert FCS1.0 and 2.0 files to text. It also adds calculated ratio, and time parameters and saves in FCS2.0. The program outputs three types of text file: raw list mode data, histogram data, and line graph data (eg mean Calcium v Time). It can handle up to 16 parameters, and allows the user to delete redundant parameters to make space. B-D vax, HP, and Mac files are opened as are Coulter IBM files. At present only Mac format FCS2.0 files are exported, but in future versions the program will save in FCS1.0 formats (LYSYS, consort 30, FACScan etc) to allow users of older systems access to ratio and time.

(Editor's note: MFI, a DOS package, also calculates and normalizes the ratio of two existing parameters, and plots any parameter vs. time or pseudo-time [event number]. It can put the results out into ASCII listmode or histogram files. Its companion program A2FCS can convert the ASCII listmode with calculated parameter into an FCS2.0 file. See also Refresh Ratio.)

Compiler/Language: Written in Think Pascal 4.0.2 for the Macintosh using ThinkClass Library version1.1. Source Code: "Too Embarrassing to Distribute". Author: Ray Hicks rh208@cus.cam.ac.uk Get FCS Assistant from the CD-ROM. Also available from the original source in the UK.

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Prints cytometer settings as recorded in files acquired with CellQuest (tm Becton-Dickinson) version 1.1 or greater, from FACScan, FACSort, or FACSCalibur instruments. Provided without warranty by its author, Ted Kunich, TedKunich@value.net. Get DropPrint from the CD-ROM. NOTE: You will need a version of Stuff-it Expander to unpack this program.

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Normalizes flow cytometric immunophenotyping data to a biological standard. This program is under development. Contact Gary Salzman, Los Alamos National Laboratory, Los Alamos NM USA. salzman@lanl.gov.

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This PC/Windows program refreshes the existing ratio of two user-specified parameters in LYSYS II listmode files (B-D FACScan, FACStar), or creates a new ratio parameter. It is untested on other FCS2.0 files. Compatible with linear or log data, 256 or 1024 channel resolution. In some instruments, the hardware/software is not fast enough to generate a correct ratio value for all events; Refresh Ratio fixes this problem.

The author is Ron Hoebe at the University of Amsterdam, Netherlands, r.hoebe@amc.uva.nl. Report problems or incompatibilities to him. (The program was written in Visual Basic 3.0 for Windows. Source code is not provided.)

Catalog editor's note: MFI (see elsewhere in this catalog) can calculate the ratio of two user-specified parameters, and rescale the result in several optional ways. Unlike Refresh Ratio, MFI calculates the ratio during analysis, and does not write it to the listmode file.

Get Refresh Ratio, or visit their home page.

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Get Programs from CD-ROM
To unpack, simply copy the program to an empty directory and execute it.

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This is a pair of programs which transfer files, including listmode files, between Hewlett-Packard (HP) Pascal 3.1 computers and PC's. Transfer can occur in either direction. Hence, PC copies of files can serve as backup which can be transferred back to the HP for analysis with HP software. PC copies of listmode files can also be used with analysis software such as WinMDI and MFI.

Transfer takes about 30 seconds per 10,000 event listmode file. Alternative transfer software sold by Verity, which uses the same hardware, is said to transfer files considerably faster. (Verity Software House, Inc., Topsham ME USA. 207-729-6767. verity@vsh.com)

Use of these programs requires an IEEE-488 GPIB card for the PC. Such cards cost about $500. One vendor which provides excellent technical support is National Instruments, gpib_support@natinst.com, accessible at the National Instruments web site. Another solution is the OptiMATE package (board plus fast commercial software) from Applied Cytometry Systems.

The PC program HPPCLINK.EXE is included in the self-unzipping file HP2PC.EXE.

The HP program HPPCLINK.CODE can also be gotten here bundled with tools which enable you to put it on an HP-formatted diskette. The instructions, in HPPCLINK.TXT, are included in the bundled program, X2FF10LK.EXE. Thanks to Scott Rouse for figuring out how to do this! (This method will allow any public domain HP program to be transferred via Internet.)

For further guidance on four possible ways to transfer files between HP's and PC's, see the file HP2PC.TXT.

These programs were provided to the public domain by Becton-Dickinson.

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This is a pair of programs which transfer files, including listmode files, between Hewlett-Packard (HP) Pascal 3.1 computers and PC's via a serial port cable. Transfer is implemented only in the HP to PC direction. PC copies of listmode files can be used with analysis software such as WinMDI and MFI.

Transfer takes several minutes per listmode file. Thus, transferring a group of files can take several hours. To make matters worse, the HPTOIBM program contains a bug which makes it transmit only every second file. After it has been run, if the first file in the series is moved elsewhere, and the program restarted, it will transfer the alternate files it skipped during the first run. Thus, the program must be run twice for every group of files to be transferred. This bug was fixed, but the fixed version of the program has not been located in recent years. In particular, inquiries with Becton-Dickinson, and with the author, Morgan Conrad, have been fruitless.

The PC program FROMHP.COM is included in the self-unzipping file HP2PC.EXE. The HP program HPTOIBM.CODE must usually be obtained by snail mail on an HP-format diskette. This is because most HP Pascal 3.1 computers do not have an ethernet port, so the diskette drive is the only way to get an external file into the computer. Ethernet ports for these computers are so expensive as to be prohibitive for most facilities. To put it another way, if your HP had an ethernet port, you wouldn't need HPTOIBM.CODE! To obtain the HP program, contact emartz@microbio.umass.edu.

For further guidance on four possible ways to transfer files between HP's and PC's, see the file HP2PC.TXT.

These programs were provided to the public domain by Becton-Dickinson.

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Resonance Energy Transfer Intensity

Calculates RETI, e.g. for donor = fluorescein, acceptor = tetramethyl rhodamine. Requires input of 12 values (4 autofluorescence, 8 donor-excited [e.g. 488 nm; 4 detected green, 4 red], 4 acceptor-excited [e.g. 514 nm]) to calculate one RETI value. Math checked by Janos Szollosi. Input batched via ASCII file. MFI (see above) was designed to generate the 12 input medians efficiently. Author: Eric Martz, Univ. Mass., Amherst MA USA. emartz@microbio.umass.edu.

If interested, request by email to have the latest version put up on the ftp server.

Get RETI by from the CD-ROM.

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Corrected Fluorescence Intensity

Accepts log-acquired median or mean intensities typed manually. Converts log values to a linear intensity scale, optionally subtracting control value and/or rescaling to MESF. C source code included. Author: Eric Martz, Univ. Mass., Amherst MA USA. emartz@microbio.umass.edu.

Get CFI program from the CD-ROM.
CFI Readme file and License.

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All the DOS programs in this collection are in the form of ZIP archives or self-extracting EXE files.
There is a free version of UNZIP.EXE here.

Macintosh programs throughout the disk are in the form of BinHex encoded Stuffit Expander archives. Some Mac programs are self-extracting (after being un-BinHexed), while others must be explicitly extracted by a StuffitExpander compatible prlgram.

Stuffit Expander is available here, but it is in BinHex format also, and you must acquire a program capable of decoding BinHex documents elsewhere.

The browsers used to navigate this CD should offer the option of downloading these files to your hard disk when the links are clicked on. In addition, you can copy the files directly from the CD to your hard disk. Each of these programs is in its own directory in the \content\software\data\ directory of the CD.

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