RE: Antwort: FACS question (P-gp)

From: Gerstein, Rachel (Rachel.Gerstein@umassmed.edu)
Date: Fri Dec 14 2001 - 18:53:29 EST


depends if you are interested in Pgp activity or expression

=======================================================
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)


> ----------
> From:		Hans-Georg.Kreysch@merck.de
> Sent:		Wednesday, December 12, 2001 11:41 AM
> To:	Cytometry Mailing List
> Subject:	Antwort: FACS question (P-gp)
>
>
>
> There are anti-P-gp antibodies conjugated to e.g. PE available from BD and
> other distributors. Possibly you could use these. (Unfortunately we had
> some major trouble when we tried the antibody from BD in a "quick and
> dirty
> experiment". I don´t know why: No p-pg expression on our cell lines or a
> bad lot of antibody?)
> Best regards
> Georg
>
>



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