Hi All, One of our users has observed an apparent doubling in the amount of DNA (based on PI staining) in the 2N population of NIH3T3 cells after selection in drug to establish cell lines that stably express a gene of interest. The cells were engineered by retrovirus. The doubling is compared to the parental cells. The selected cells have a 4N population with 4 times the DNA of parental 2N cells. Has anyone ever seen this. Is it likely to be some artifact of staining? Thanks in advance. Ronald J. Hill, Ph.D. Sr. Group Leader, Research Sugen, Inc. 230 East Grand Ave. South San Francisco, CA 94080 ron-hill@sugen.com tel: 650-837-3797 fax: 650-837-3313
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