Laura, I've seen calcein AM excluded from P-glycoprotein positive cell types in a live/dead assay for testing drugs against AML patient samples. Calcein give s a very bright green signal that you could use against FL2-3-4. This, and others, are discussed more at Mol.Probes site. (link to < http://www.probes.com/handbook/sections/1505.html >) MAK. >>> Laura Hodges <lmhodges@yahoo.com> 12/10/01 02:58PM >>> I'd like to post a question on your website: Can anyone suggest another P-gp accumulation probe other than rhodamine 123 which has too broad of an emission spectrum for my use. I am trying to find a P-gp probe that can be used with other fluorescent markers in channels FL2, FL3, and FL4 in a multicolor assay. __________________________________________________ Do You Yahoo!? Send your FREE holiday greetings online! http://greetings.yahoo.com
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