Laura- Rh123 emission is a little longer than FITC, but it can still be used adjacent to PE with a standard filter configuration. We have done a considerable amount of 4-color work using Rh123, PE, ECD and PECy5 (see Donnenberg VS, Burckart GJ, Griffith BP, Jain AB, Zeevi A, Donnenberg AD. P-glycoprotein (P-gp) is upregulated in peripheral T-cell subsets from solid organ transplant recipients. Journal of Clinical Pharmacology 2001; 41:1271-1279). Albert and Vera Donnenberg University of Pittsburgh
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