El 30/11/01 18:03, "Andrea Illingworth" <dcdsflow@mint.net> escribió: > Dear group, > What are your thoughts on the use of CD40 to differentiate normal plasma cells > from myeloma cells? > Currently we are using the CD45/CD56/CD38 combination but we are looking into > either adding CD138 and/or CD40. Would you recommend one over the other or > recommend adding both antibodies? > > Thank you for your input! > > Andrea Illingworth > Dahl-Chase Flow Cytometry > Bangor, Maine 04401 > Andrea_Illingworth@dahlchase.com > > Dear Andrea: > In our lab we are using CD19 and CD138 to differentiate normal plasma cells > and myeloma cells. The combinations are: CD38/CD138/CD45, CD38/CD56/CD19, > CD54/CD20/CD56 and cytoplasmic Kappa/Lambda in combination with CD38 or CD138. > Usually we don´t have problems to distinguish normal and abnormal plasma > cells. > Actually we using CD138 performed by Immuno Qualyty Products Cat. Number > IQP142F (FITC) and IQP142R (PE) > Best wishes > Dr. Mariano P. Scolnik IO/IIHEMA Academia Nacional de Medicina Pacheco de Melo 3081 (1425) Buenos Aires Argentina Tel: (54-1) 4805-5759 Ext 289 FAX (54-1) 4803-9475 > > >
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