Eric, The time delay does not eliminate the overlap, you will still need to run compensation controls and do the compensations. Randy T. Fischer NIH/NIAMS Autoimmunity Branch Building 10, Rm 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Nealley, Eric W Mr USAMRICD > Sent: Wednesday, November 21, 2001 9:55 AM > To: Cytometry Mailing List > Subject: 3 color combination? > > > We are trying to run a 3-color experiment on our FACScalibur (with FL4 > option) and I was concerned about spectral overlap between propidium > iodide > and APC. We want to use annexin V FITC, propidium iodide and CD3 APC. I > know there is a time delay procedure to calibrate the instrument, but does > the spectral overlap between propidium iodide and APC get "cancelled out" > by > the time delay? Our instrument setup: FL1: 530/30, FL2: 585/42, FL3: > 670/LP and FL4: 661/16. > > Thanks in advance. > > Eric Nealley > > > >
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