3 color combination?

From: Nealley, Eric W Mr USAMRICD (Eric.Nealley@apg.amedd.army.mil)
Date: Wed Nov 21 2001 - 09:55:13 EST

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We are trying to run a 3-color experiment on our FACScalibur (with FL4
option) and I was concerned about spectral overlap between propidium iodide
and APC.  We want to use annexin V FITC, propidium iodide and CD3 APC.  I
know there is a time delay procedure to calibrate the instrument, but does
the spectral overlap between propidium iodide and APC get "cancelled out" by
the time delay?  Our instrument setup:   FL1: 530/30, FL2: 585/42, FL3:
670/LP and FL4: 661/16.

Thanks in advance.

Eric Nealley

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