I would like to ask any of you to provide expertise for my clients on cultured bone marrow derived dendritic cell analysis by flow cytometers. We found the background was very strong using unstained cells. Therefore, we had to lower the voltage of PMT(FL1, FL2) to approximately 420-250. However, the negative area of dot plot FL1 vs FL2 showed narrow elliptical shape toward diagonally. It did not show the round or oval shape as many other white blood cell clusters did.The antibodies were used - CD11c-PE(data.oo2) and CD86-FITC(data.003). Data.001 is unstained sample for negative control. The staining buffer is PBS with 1%FBS and 0.05% NaN3. Please advise. Thank you very much. Yen-Chi Chen BD Taiwan, BDB-COE Tel: 02-2722-5660 ext 297 Fax: 02-2725-1768 yenchi_chen@bd.com (See attached file: Data.003)(See attached file: Data.002)(See attached file: Data.001)
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