Hi Everyone,
If you have a 0-10 PSI pressure gauge with a set of quick disconnect
fittings, measure the sheath tank pressure. It should be 4.5 PSI. If it is
not 4.5 PSI adjust the regulator, located in the lower right portion of the
instrument, for 4.5 PSI. Once 4.5 PSI sheath tank pressure has been
established, measure the sample tube pressure. This can be measured at green
line going into the flow cell. A ball park measurement should be 4.1 PSI for
low, and 4.6 PSI for high. Sheath fluid pressure will be lower at your flow
cell than in your sheath tank. Your flow rate in high should be 60uL/min and
in low, 12uL/min. If you would like a pressure gauge contact me. Hope this
helps.
Ray
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: Marco A. Fernández <marcoa@ns.hugtip.scs.es>
To: cyto-inbox
Date: Friday, October 26, 2001 3:42 PM
Subject: Sample pressure
>
>Dear Flow'ers,
>
>We have started a discussion in our lab regarding sample pressure vs.
sheath
>pressure in FACS benchtops (we have a FACScan). I am not sure but I think
>that sample pressure is always over sheath tank pressure (4.5 psi for
sheath
>and 4.6 -LO- and 5.0 -HI- for sample). Is that right?
>
>Best regards,
>
>
>Marco A. Fernández
>FACS Facility
>University Hospital Germans Trias i Pujol
>08916 Badalona (Barcelona)
>Spain
>marcoa@ns.hugtip.scs.es
>http://citometria.furebtip.org
>
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