If you want to squeeze your sample into a flow cell with sheath fluid pushing through it, you will need to use more pressure on your sample to overcome the sheath pressure - otherwise no sample gets in and no data comes out. Simple as that. A negative pressure differential (sample vs sheath) would mean that your sample would quickly get diluted out as the sheath fluid oozes into your carefully prepared sample. You might encounter some bright whip who thinks that a venturi effect is enough but my guess is that you would be running at very slow data rates. At 05:11 PM 10/25/01 +0200, Marco A. Fernández wrote: >Dear Flow'ers, > >We have started a discussion in our lab regarding sample pressure vs. sheath >pressure in FACS benchtops (we have a FACScan). I am not sure but I think >that sample pressure is always over sheath tank pressure (4.5 psi for sheath >and 4.6 -LO- and 5.0 -HI- for sample). Is that right? > >Best regards, > > >Marco A. Fernández >FACS Facility >University Hospital Germans Trias i Pujol >08916 Badalona (Barcelona) >Spain >marcoa@ns.hugtip.scs.es >http://citometria.furebtip.org Derek Schulze Flow Cytometry and Confocal Microscopy Facility Manager Cancer Research Labs Queen's University Kingston, ON Canada
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