Flowers For all those who gave me suggestions for the 6 colour combination ..... thankyou. After checking a few sources of APC-Cy7 and switching around PMTs we were able to get a very bright signal (>2 log shift) which is more what was expected. Also Alexa 350 gave a poor (0.5 log shift) with the UV, but this was expected from what people were saying ..... this may not be used in the future. The combination we plan to work with are: FITC PE ECD PE-Cy7 APC APC-Cy7 .....this still gives us the opportunity to play around with UV fluorochromes. Now is the next question......we need an anti-Rat IgG in one of the less common colours (APC-Cy7, PE-Cy7 or PerCP5.5). Does anybody know of a good source ?? Andy Biomedical Research Centre UBC FACS Facility Manager Vancouver
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:36 EST