I have been reading with interest this thread on good vs. bad data. I consider myself a fairly good flow cytometer/sorter operator with a good grasp on the theory and practice of the art/science. I provide advice and resources for my users on how to set up their experiments and how to analyze their data. However, when it comes to publishing their data, having never published flow cytometry data myself, I wouldn't know what kind of advice to give them. In fact, I was surprised this was an issue. I would be very interested in some kind of guide to hand them on what is acceptable and what is not. So, in a nutshell, if someone could whip up some guidelines to post on this site, I would find that very useful. I know, from this thread, that task would not be simple and there would be some dissension on what is and is not essential, but it would at least give us operators somewhere to start. It may be that there is no one set of rules that will govern every experiment. We, for example, do experiments with bacteria and parasites. If that is the case, I will continue to give the best advice I can on what I think is necessary to prove a point and continue watching this site for any advice I can glean for my researchers. Thanks for your help in advance, Julie ---------Included Message---------- >Date: Tue, 23 Oct 2001 09:48:42 -0400 >From: "DARZYNKIEWICZ ZBIGNIEW" <DARZYNK@nymc.edu> >To: "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu> >Subject: Re: Bad Flow Data & reviewing -- What can we do? > > >To the ongoing discussion on bad data let me add my comments. >First, I fully concur with all Paul Robinson's conclusions. The one word >missing at the end was: "Amen" >To help the authors to present their data in a meaningfull way and extract >as much information as possible, I propose that the group of the most >experienced colleagues will develop a page or two flier - Instructions to >the authors - How to present your flow cytometric data. This has to be a be >very thoughtfuly developed document. The input to it should be sought from >many experts in fields of immunology - cell cycle - apoptosis - graphics - >statistics, perhaps even estethics. We have many such experts in the >Editorial Boards of these journals. Howard Shapiro, Alice Givans, Mike >Ormerod have already a lot of experience while preparing illustrations to >their books. Once such document is prepared and agreed it should be >distributed not only to Cytometry or CCC but to other journals as well >(Cancert Res, Science, Immunology) etc, with the request to the editors to >include it to their "Instructions to Authors". >I will be glad to co-ordinate this effeort - I will ask Paul Robinson and >Harry Crissman as Co-editors. We will then co-opt other colleagues and start >the work. I envisage that in the course of our work we present the >developing instruction to this forum asking for comments. If you think its >is a good idea - plese let me know. > > > Zbigniew Darzynkiewicz >Brander Cancer Research Institute >New York Medical College >19 Bradhurst Ave. >Hawthorne, NY 10532 >tel: 914-347-2801 >fax: 914-347-2804 >http://www.geocities.com/z_darzynkiewicz > > > ---------End of Included Message---------- Julie G. Nelson Research Coordinator Flow Cytometry Facility Center for Tropical and Emerging Global Diseases University of Georgia
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:36 EST