We perform 34 positive selections on PB and bone marrow quite often. On a good day our purity is better than 90%. The beads do not affect the FSCxSSC of the recovered cells. To check the purity be sure to use an 34 antibody to a different epitope. I believe the Miltenyi 34 kit uses an antibody to HPCA-2 for selection, therefore, you should use an antibody to HPCA-1 to check your purity. -- Paul Weiss Flow Cytometry Specialist University of Illinois at Chicago 900 S Ashland Ave Chicago, IL 60607 Phone: 312-355-0123 Fax: 312-413-7963 pweiss1@uic.edu > From: "Kong, Denice" <KongD@surgery.ucsf.edu> > Date: Thu, 18 Oct 2001 14:00:46 -0700 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Miltenyi magnetic beads and CD34+ > > > > Dear flow users: > > I am currently evaluating using Miltenyi microbeads to isolate CD34 from > peripheral blood and checking the purity using flow. Has anyone done this > type of study before. If so, where would the CD34+ cells be on the SSC and > FSC plot with or without the microbeads? What purity was obtained? > > Denice Kong > UCSF-ITL >
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