Hi Geoff, This has come up a couple of times over the years for plain old CellQuest, and I had a quick look through a couple of files when Chris groves asked in May 1998, and I asked at the time if anyone from B-D would cast any light on it - if they have, they haven't shone it in my direction. I don't know if things have changed for Pro, or if these things are valid for sorters (presumably they won't hold for machines with more than 5 PMTs), but this is what I gleaned (and Chris confirmed) back then: > BD$WORDS 14-18 cover PMT volts for ssc to FL3, BD$WORD 13 looks like it > might encode FSC gross gain (E00,E01,E02,E03,E-1 being covered by 0 to 4). > > BD$WORDs 18-22 have 100 times the incremental linear gain (eg 5.57 maps to > 557). > > 23-27 seem to indicate log/lin amplification (1 for lin, 0 for log). > > 29 to 33 seem to cover the trigger thresholds for parameters 1 to 5. I > haven't got a compensated sample so I can't help you out on that front, but > my guess is that it would be held in words 34 to 37 (as 10 times the value) > presuming that there are four potential compensations in the file I've got. I haven't revisited this stuff since 1998, but will do next week and see how it works for the Calibur that I have. If you'd send me a couple of files acquired with different settings from the same machine, along with a print-out of the settings in use at the time, I'd be happy to pick through them and tell you what I come up with. Chris told Alice Givan of DropPrint when she asked a similar question in September 1999, the link that Alice gave on Eric Martz's site appears to have died, but a big up to Purdue for pulling together all things flow cytometric : http://www.cyto.purdue.edu/flowcyt/software/Catalog.htm#dropprint It crashed when I tried it out just now, which doesn't mean much, except I can't tell you what it produces, so you might want to try it out yourself. Cheers, Ray ps Maciej, you can use this to your web page if you'd like.
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