Greetings all, My question pertains to DNA cell cycle analysis in frozen tumors. By definition, tetraploid peaks have DI's from 1.9 - 2.1 with the proportions of cells greater than that of the normal diploid G2/M population. In samples, when the diploid S phase is usually low, I would expect a somewhat low G2/M as well, but when I see a G2/M of 40% I immediately think tetraploid. Is there a clear cut way to distinguish and validate normal G2/M cells vs. Tetraploid populations? I ask for your enlightenment. Richard A. Salinas Flow Cytometry Laboratory Dept. of Radiation Oncology Texas Research Park San Antonio, TX 78245 (210) 677-3882
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