Advice on 4 colour flow

From: linda duncan (opt059@abdn.ac.uk)
Date: Thu Oct 18 2001 - 14:50:30 EST


Dear All,
For various reasons, I will be purchasing antibodies from
Caltag, and need to get a combination that will work. I
know that I can use FITC/PE/PerCP/APC but cannot use
FITC/PE/Cychrome/APC because of the overlap in excitation
between Cychrome and APC,but Caltag has three different
fluorochromes on FL3: PE-TR; PE-CY5; PE-CY7. Which FL3
Fluorochrome from Caltag will work with FITC, PE,and APC?
More importantly which ones won't work. Please help!!

----------------------
Linda Duncan
Dept Ophthalmology
University of Aberdeen
Medical School
Foresterhill
Aberdeen
AB25 2ZD
l.duncan@abdn.ac.uk



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