We get CSF specimens like this all the time. I feel more comfortable when I know the immunophenotype of the tumor from lymph node, etc, but this isn't always possible. What was the lambda staining. If you have CD19+ cells that are all positive for kappa and negative for lambda then you have a monoclonal B cell population (as long as this isn't the non-specific diagonal pattern or due to the kappa reagent being brighter than the lambda reagent resulting in false apparent dim staining). In an HIV lymphoma that means treat them here. If you only add kappa and don't have lambda- you can't interpret it. Maryalice > ---------- > From: cy > Sent: Sunday, October 14, 2001 3:02 AM > To: Cytometry Mailing List > Subject: CSF > > Hi everyone, > > We received a CSF specimen for flow. Cell count was 5/ul. The sample was > from a lymphoma patient with HIV positive. The physician would like to > know if CSF was involved. > > We acquired as much cells as possible but the number of CD45 positive cell > was still very low (87 events). About half of them (36 events) were CD19+ > B-cell. Of these, 29 events were positive for kappa. How significant are > these numbers? Would you call this CSF involvement with kappa > restriction? How would you have reported this? > > Your opinion will be very much appreciated. Thanks. > > cy > > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:34 EST