Re: Multi level beads for linearity, etc

From: craig.turner@nbs.nhs.uk
Date: Mon Oct 15 2001 - 02:45:56 EST


>
>Hi All,
>I have a B/D FACScan and two Coulter XLs.  I'm looking for the
quintessential multi-level
>fluorescent bead product to do linearity and sensitivity, compatible with
both Coulter
>and B/D.
>Thanks in advance
>
>Hello Deborah,
>I have used Sphero rainbow calibration particles from Spherotec inc. (cat
>no. RCP-30-5A) on our ELITEs with success they have 8 fluorescence peaks
>(of which I can resolve 6 on a good day). I have used them as a one-off to
>check linearity/sensitivity and have also 'spiked' them into samples as an
>internal standardisation control -if anyone would like a copy of the excel
>formula that processes the values they can contact me directly.
>Best regards,
>Arnold.

Hi all,
I wouldn't hold this as total gospel, but I was discussing the need for some beads
with a BD rep a few months back, and he indicated that BD rainbow beads are sourced
from spherotec. Can anyone confirm this?
Craig



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