Hi everyone, although not strictly a flow question, my query relates to staining cells with fluorescent antibodies so... We are trying to look at freshly isolated murine lymphocytes by confocal microscopy. We would like to look at both cell-surface and intracellular antigens, and need some advice. Let's say we want to stain for CD3 : 1. Should you stain the cells in a tube, fix, and then try to make the cells stick to a coverslip ? 2. Or have the cells adhere first, stain and fix ? 3. What type of mounting media / coverslip ? Hope someone is able to help us Greetings, Oberdan
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