HI All A number of you have responded to my question about this a week or two ago. . .Thanks. But it seems all this might be impossible if you're doing cell cycle analysis. Has anyone actually grown enough adherent cells in 96 well format in order to trypsinize and fix and stain all in the plate and do your analysis from that same plate? - Assuming you want to analyze at least 10,000 events - after gating out other stuff. I've always started with a lot more cells than I grow in 96 plate wells. Also, even if you grow in other plates and transfer, the cells would have to be at high concentrations and run slowly - so it would take a long time. Somebody mentioned running a plate in 45 minutes - this is for what sort of analysis? Also, is the 96 well attachment easily interchangable with a carousel or just a single tube loader??? Thanks much. Ellen Freed Ellen Freed Scientist AstraZeneca R&D Boston
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