Hi, Just wondering if anyone has experience assessing the specific oxidation of 2,7-diaminofluorene (DAF) by pseudoperoxidase activity of hemoglobin (Hb)to generate fluorene blue (FB) by flow cytometry? There seems to numerous plate reader based assays i.e. colourimetric, but I haven't been able to find out if the fluorene blue is at all fluorescent. Any advice appreciated. Thanks Geoffrey Osborne Specialist, Flow Cytometry, John Curtin School of Medical Research, The Australian National University, Canberra, 0200, ACT. AUSTRALIA email: geoff.osborne@anu.edu.au http://jcsmr.anu.edu.au/facslab/facshome.html (61 2) 6125 3694.
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:33 EST