I just considered both the Beckman/Coulter Instrument and the FACSCalibur and made the decision to purchase the BD instrument. While I thought that the digital electronics of the Coulter machine were better, I could not live with the fact that I would be stuck with a single argon laser. The Diode laser on the BD instrument allows me to use a number of fluorochromes and dyes that simply cannot be used on the Coulter. The tandem dyes work well if one is aware of their caveats regarding comopensation and lot to lot variability, but why use them if you don't have to? James James F. George, Ph.D. Division of Cardiothoracic Surgery Department of Surgery University of Alabama at Birmingham Birmingham, AL 35294-0007 Phone 205-934-4261 FAX 205-934-5261 jgeorge@uab.edu ----- Original Message ----- From: "Houston, Jim " <Jim.Houston@stjude.org> To: cyto-inbox Sent: Thursday, September 27, 2001 9:11 AM Subject: RE: 4 color capability of Beckman CoulterEPICS XL > > I do not know about the coulter but I have a BD LSR that does this rather > well. > We are able with no problems using the standard FITC-Pe along with > PE-TR(ECD) as the third color and either PerCP or PE-CY5 as the fourth > color. > Compensation controls are available within CellQuest to compensate even FITC > vrs. PE-CY-5. > Works well in our hands. > > Jim Houston > > PS:This is not a BD commercial. > > > ---------- > > From: Delano Young > > Sent: Wednesday, September 26, 2001 8:17 AM > > To: Cytometry Mailing List > > Subject: 4 color capability of Beckman CoulterEPICS XL > > > > > > Folks, > > > > Does anyone have experience with the EPICS XL's 4 color capability using > > just one laser? How does it compare with 4 color usage using the standard > > 2 > > lasers (e.g., the FACSCaliburn 2 laser system)? > > > > Respond to dyoung@nitromed.com or 781-685-9784. > > > > Del > > > >
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