Hi, With the remark I received concerning UV laser reliability in the LSR (see below) I was wondering if anyone could comment on Cytomations claim that their UV laser in their new Cyan machine has less noise and whether the LSR could be retrofitted if need be with a less noisy HeCd. Gene/UCONN Health If you used an LSR be aware that those lasers won't allowed you to performed cell cycle measurements with reasonnable CV for all there life time. In our hands, usually you can perform DNA content and cell cycle for a period of about 8 to 12 months(wich is in accordance with HM Shapiro, Practical flow cytometry).Those lasers became noisy after only a few months and getting worst as time goes by.For calcium flux they are perfect because with Indo-1 you will do a ratio measurement wich eliminated the noise problem. Marcel Manager, Core cytometry lab. INRS-Institut Armand Frappier Université du Québec
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