Lipofectamine auto fluorescence

From: Tami Rosario (trosario@cellmate.cb.uga.edu)
Date: Fri Aug 31 2001 - 15:52:05 EST


We are transfecting COS 7 cells with a plasmid containing GFP using
lipofectamine.  We see a large amount of auto fluorescence from the
lipofectamine alone in our controls.   Has anyone else experienced
this?  If so, how can we remedy the situation.
Thanks for your help,
Tami Rosario
Department of Cellular Biology
University of Georgia



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