ERRATUM: I would just like to correct my misconception about the cellular restriction of MHC class I molecules, as Ian Nicholson just mentioned to me: "Just thought I should point out (with respect) that human platelets do in fact express class 1 Ags. Indeed, in some situations there is a clinical need for HLA matched platelets (class 1) where patients generate anti-HLA Abs." Cheers Ian Nicholson Victorian Transplantation and Immunogenetics Service" Thanks for the correction, Ian Karim Vermaelen wrote: > In humans the main MHC class I subfamilies are HLA-A, HLA-B and HLA-C > (e.g. HLA-B27): these are present on virtually all cells of the body > except RBC and platelets. > The main MHC class II subfamilies are HLA-DP, -DR and -DQ (e.g. > HLA-DR4). MHCII molecules are only expressed on APCs (DCs, B cells, > M-phages, thymic epithelium) and sometimes in other cells under > certain inflammatory conditions. > > In fact the HLA nomenclature refers to the gene encoding for the MHC > molecule. Every individual has all these genes, but each one in a > specific "version". Some "versions" are typically linked with a risk > for certain auto-immune diseases (e.g. HLA-B27 in spondylarthritis, > HLA-DR4 in rheumatoid arthritis). HLA-A,B,C version typing is also > used to determine how far donor and acceptor tissue match prior to an > organ transplantation. > > Traditionally, to detect MHCII on APCs, one uses an anti HLA-DR > antibody (we use the ones from BD; they have them in FITC, PE, PE-Cy5, > APC and biotin). > > Hope this basic info will help you somewhat > Karim > > Maciej Simm wrote: > >> Dear group, >> >> I'm trying to read up on the topic of antigen presenting. One of the >> >> topics that gives me trouble is the nomenclature. What I know and >> learned about as MHC class 1 and MHC class 2 are often referred to >> as >> HLA in reagent catalogs. >> >> I was looking for reagents that would distinguish between MHC I and >> II and found HLA class I and that was easy. But it seems there are >> many reagents for HLA class II >> >> My question, - what if any, is/are a good general reagent/clone >> that >> would detect MHC class II for professional APC's ? >> >> thanks for any insights, >> >> Maciej >> >> __________________________________________________ >> Do You Yahoo!? >> Make international calls for as low as $.04/minute with Yahoo! >> Messenger >> http://phonecard.yahoo.com/ >
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