Maciej, "HLA" refers to the human MHC (class I or class II) loci or antigens. These include DR, DQ, and DP, plus a few other "non-classical" class II antigens. Pharmingen offers a reagent that recognizes all DR and DP alleles and some DQ. Either this or the DR-specific reagents will stain class II positive cells, which will include B lymphocytes as well as "professional" APC. You may therefore need to use a marker that is more specific for macrophage/DC's than is class II. Good luck, Mandy Cromwell >Dear group, > >I'm trying to read up on the topic of antigen presenting. One of the >topics that gives me trouble is the nomenclature. What I know and >learned about as MHC class 1 and MHC class 2 are often referred to as >HLA in reagent catalogs. > >I was looking for reagents that would distinguish between MHC I and >II and found HLA class I and that was easy. But it seems there are >many reagents for HLA class II > >My question, - what if any, is/are a good general reagent/clone that >would detect MHC class II for professional APC's ? > >thanks for any insights, > >Maciej > >__________________________________________________ >Do You Yahoo!? >Make international calls for as low as $.04/minute with Yahoo! Messenger >http://phonecard.yahoo.com/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Mandy A. Cromwell, Ph.D. Research Fellow Harvard Medical School Department of Immunology New England Regional Primate Research Center One Pine Hill Drive Southborough, MA 01772 (508)624-8022
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