Yes. mr At 6:47 PM -0400 8/29/01, John Altman wrote: >Mario, > >Do you do this for your bench top analyzers as well? Might the NaOH damage >the quartz cell? > >John > >> >> We have recently become very fond of using 0.1N NaOH to "cleanse" our >> cytometer. The advantage is that it hydrolyzes DNA/RNA, which is one >> of the major contributors to clogs for certain cell samples. It >> solubilizes cells quite well. >> >> After any clog (partial or full), and at the end of the day, we >> always run three things through the cytometer (make sure the fluid >> from each runs long enough to go through the nozzle--i.e., boost for >> 15-30 seconds minimum). >> >> (1) 0.1N NaOH (sterile filtered, made in the best water you have) >> (2) CoulterCleanse solution >> (3) dH2O (if end of day, we leave the tubing filled with distilled water) >> >> After each of the tubes 1-3, we wipe down the outside of the sample >> inlet tube with a kemwipe, while the instrument back-flushes sheath >> fluid, and wash with dH20, to prevent contaminating future samples >> with the base or the cleansing solution. >> >> To sterilize, we may also run 70% ethanol through the system. >> >> However, I strongly endorse the NaOH--It cleans clogs like nothing >> else we've ever tried. >> >> mr >> >> (Disclaimer: I have no idea what 0.1N NaOH might do to your tubing, >> nozzle, or other equipment. We've used it for nearly a year with no >> problems on multiple instruments, however, make sure you understand >> the risks to your instrument of any solution you apply to your >> fluidics). >> > > > >*************************************************************************** >John D. Altman, Ph.D. >Emory University Vaccine Center at Yerkes >954 Gatewood Road >Atlanta, GA 30329 > >Office: (404) 727-5981 >FAX: (404) 727-9005 >FAX2: (404) 727-8199 >Lab: (404) 727-8914 > >email: altman@microbio.emory.edu > >*************************************************************************** >Tetramer Core Facility: >http://www.niaid.nih.gov/reposit/tetramer/index.html >***************************************************************************
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