Hi Group,
Given the recent discussions on the formation of a
bacterial flow cytometry group this posting will hopefully fall on helpful
ears. I am trying to study the microbial ecology of the deep subsurface of
the Wits basin in South Africa. I have collected 50-70C water samples from
between 2 and 3.6km deep and believe it or not, FC and DNA staining reveals
microbial counts of up to 10e5 cells per mil in some samples. I want to use
FISH to gain an insight into the phylogeny of the samples and wondered if
anyone has any preservation protocols for environmental samples prior to
FISH and then FC analysis. Thanks in advance and I appreciate any feedback
as I am a little isolated in terms of libraries or journals!!! Cheers,
james
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James Hall, Ph.D. In Glenharvie, SA from April
22nd-August 6th
Dept. Geosciences, (phone) 011 27 11 752 2520
Princeton University, (mobile) 011 27 83 993 0119
Princeton, NJ, 08544. hall@princeton.edu
(phone) 609-258-2597
(fax) 609-258-1274 Check out the field project
web site,
"err, I guess it is okay"
Internet Imaginary
http://home.mweb.co.za/ja/jahall
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