Amy, I don't know any markers that will stain _only_ monocytes. Here are some we use: CD4, (easily shed upon activation, also expressed on T cells) CD11c (but also B cells from HCL patients) *CD14, (stains poly's too) *CD33, (except after PMA activation, also stains granulocytes) CD68, (not very exclusive to monocytes, esp. in intracellular work) CD74, (but rather macrophages) I also found some funky mono/myelo CD markers here: http://www.researchd.com/rdicdabs/cdindex.htm some other helpful links: http://www.ncbi.nlm.nih.gov/prow/guide/45277084.htm http://129.106.232.8/pinnacle7/CdMarkerDX/MarkerList_1.asp I've been campaigning monocyte "definition" on this group before and decided to use at least 2 fluorescent markers and side scatter as a minimum criteria (ex. CD14and/or33 + CD45 + SSC). I find the "legacy" method (ie. side scatter vs. CDXX) does not work as well in activated cells. The most important thing however is to pick one staining protocol and stick to it :) Good luck, Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Get personalized email addresses from Yahoo! Mail http://personal.mail.yahoo.com/
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:25 EST