In addition to the below, CD64 is a good monocyte marker (although is unregulated on PMNs in sepsis or a systemic acute inflammatory response. CD163 is the only monocyte specific marker I am aware of and in a preliminary study we have found this detects more monocytes than CD14 does. One source of both CD64 and CD163 is Maine Biotechnology Services (www.mainebiotechnology.com). The best method for counting monocytes may well be a combinations of CD14, CD64, and CD163. Regards, Bruce H. Davis, M.D. Maine Medical Center Research Institute 81 Research Drive Scarborough, Maine 04074 USA PHONE: 207-885-8113 FAX: 207-885-8110 Email: davisb@mmc.org >>> Maciej Simm <simmmmer@yahoo.com> 07/10/01 06:12PM >>> Amy, I don't know any markers that will stain _only_ monocytes. Here are some we use: CD4, (easily shed upon activation, also expressed on T cells) CD11c (but also B cells from HCL patients) *CD14, (stains poly's too) *CD33, (except after PMA activation, also stains granulocytes) CD68, (not very exclusive to monocytes, esp. in intracellular work) CD74, (but rather macrophages) I also found some funky mono/myelo CD markers here: http://www.researchd.com/rdicdabs/cdindex.htm some other helpful links: http://www.ncbi.nlm.nih.gov/prow/guide/45277084.htm http://129.106.232.8/pinnacle7/CdMarkerDX/MarkerList_1.asp I've been campaigning monocyte "definition" on this group before and decided to use at least 2 fluorescent markers and side scatter as a minimum criteria (ex. CD14and/or33 + CD45 + SSC). I find the "legacy" method (ie. side scatter vs. CDXX) does not work as well in activated cells. The most important thing however is to pick one staining protocol and stick to it :) Good luck, Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Get personalized email addresses from Yahoo! Mail http://personal.mail.yahoo.com/
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