PBMC volumes via Z1 Particle Counter

From: Gaffin, Stephen L USARIEM (Stephen.Gaffin@na.amedd.army.mil)
Date: Wed Jul 11 2001 - 07:53:15 EST


Hello Flowers,
	I am trying to establish the best Lower Volume Gate in my Coulter Z1
for determining the concentration of isolated human PBMCs.

	Coulter cannot provide a value.

	My preliminary correlations with hand counting suggest	60-65 fl.
Does anyone have another, better value?

Thanks,  Steve
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** Stephen L. Gaffin, Ph.D.				   **
** Research Physiologist				   **
** U.S. Army Research Institute for Environmental Medicine **
** Natick MA  01760-5007				   **
** Tel. 508-233-4867	   Fax 508-233-5298		  **
** email  stephen.gaffin@na.amedd.army.mil		   **
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