Steve Gaffin wrote- > I am trying to establish the best Lower Volume Gate in my Coulter Z1 >for determining the concentration of isolated human PBMCs. > > Coulter cannot provide a value. > > My preliminary correlations with hand counting suggest 60-65 fl. >Does anyone have another, better value? 65 fl is less than normal RBC volume. My recollection is that lymphocytes are 180-200 fl and granulocytes and monocytes are bigger. -Howard
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