Dear Parisa, I am also interested in Fc receptor blockade and would appreciate hearing what other investigators are using (besides Fab fragments and FcR specific monoclonals). I predominantly work with platelets and have found that some monoclonals augment platelet activation via the FcR. Thanks, Lori Krueger Center for Platelet Function Studies UMass Medical School ----- Original Message ----- From: "Amjadi, P" <p.amjadi@ic.ac.uk> To: cyto-inbox Sent: Friday, June 22, 2001 10:36 AM Subject: FC block in immunophenotyping of human leukocytes > > Dear All, > We have observed quite a high background staining when we use fluorochrome > conjucated mAbs for immunophenotyping of human machrophages and dendritic > cells. I am looking for a FC block reagent in order to reduce the > background staining. I checked the Pharmingen/BD catalogue and they only > provide the reagent for rat and mouse and not for human. I would appreciate > if someone could help me with this query. > > Regards > Parisa
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