Dr. Gilles Devouassoux inquires: " I want to analyze the eosinophil population from PBMC and sputum using fax. > Several selection markers have been described. What is the best > technique!!!!! Any suggestion will be appreciated " > A simple and perhaps the least expensive method to detect easinophils utilizes FITC. This reagent has approximately 1000 times higher affinity to label eosinophils than any other blood cells (Bedner et al., Cytometry, 36: 77-82, 1999). Thus, at concentration between 2 and 500 nM FITC can be used a specific marker of human eosinophils. Most likely FITC binds to the eosinophil granule major basic protein. It is unknown whether it can also may be used to selectively label mice or other animal species eosinophils. It should be noted, however, that eosinophils have strong blue autofluorescence upon UV light illumination which also can be used to distinguish them, at least from other blood cells. I expect that the the companies that sell monoclonal antibodies as markers will try to dissuade the use of FITC as they prefer to sell rather more expenive products. Zbigniew Darzynkiewicz Brander Cancer Research Institute New York Medical College 19 Bradhurst Ave. Hawthorne, NY 10532 tel: 914-347-2801 fax: 914-347-2804 http://www.geocities.com/z_darzynkiewicz "
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