Storage of Lymph Nodes

From: Munsch, Margaret (mamuns@pahosp.com)
Date: Thu Jun 28 2001 - 15:38:45 EST

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Please forgive these basic questions from a new flow person regarding the
best way to store lymph nodes for Leukemia/Lymphoma evaluation when the node
comes in late in the day and must be kept for the following morning.  Should
I place the intact node in RPMI or should I go ahead and collect the cells
from the node and place them in RPMI?  Should I then place the node and/or
cells in the refrigerator or leave out at room temperature?  Thank you.

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