Ca2+

From: Fjerdingstad, Hege (Hege.Fjerdingstad@no.nycomed-amersham.com)
Date: Mon Jun 25 2001 - 07:16:04 EST 

Hi!!
I have some problem measuring Ca2+ with my Epics Elite.  I load the cells
for 45 min with Fura red and Fluo-3.  After washing the cells I run them
with an argon 488 nm laser.  I try to stimulate both ECV-304 cells and MNC
with PHA / FMLP or histamin without seeing anything.  I'm looking for the
ratio between Fura red	and fluo-3 against time.  Do anyone out ther have a
procedure for staing and also for doing the calculation??
Regards
Hege Brincker Fjerdingstad

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