I'd have to agree with Andy. There are several good off-line programs out there. Verity Software's WINLIST program has countless features that we find invaluable for list mode analysis including batch analysis, auto-compensation, export/import functionality, spreadsheet linking, database support...the list of features is extensive. And, it's very easy to learn. CellQuest is fine for lots of app's but flexability and learning seem to be easier with these alternative programs. As mentioned in an earlier discussion, check around and try some demos before jumping in. My two cents, Bruce Greig Immunopathology Lab Vanderbilt Univ. Medical Center Nashville, TN.
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